rabbit anti arp2 3 subunit 1b antibody Search Results


rabbit anti arp 2 3 complex antibody  (Bioss)
92
Bioss rabbit anti arp 2 3 complex antibody
Rabbit Anti Arp 2 3 Complex Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actin anti actin mouse  (Proteintech)
93
Proteintech actin anti actin mouse
Actin Anti Actin Mouse, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arp2 3 complex  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology arp2 3 complex
Arp2 3 Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arpc3  (Proteintech)
93
Proteintech arpc3
Arpc3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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capzβ  (Proteintech)
93
Proteintech capzβ
Figure 1. <t>CapZ</t> is associated with early endosomes. (A) CapZβ-GFP-expressing HeLa cells were immunostained <t>with</t> <t>antibodies</t> against EEA1 (top panel) or RAB5A (lower panel). Confocal images taken along the z-axis were projected (Maximum Intensity Projection). The colocalization coefficients (MCC) of CapZβ-GFP and EEA1 or CapZβ-GFP and RAB5A were quantified. (B) CapZβ-GFP/RFP-RAB5A-expressing HeLa cells were treated with or without YM-201636 (5 μM) for 8 hr, followed by confocal imaging. The colocalization coefficients (MCC) of mRFP-RAB5A and CapZβ-GFP were quantified. (C) The N-SIM S Super-resolution imaging of CapZβ-mCherry/RAB5A-GFP-expressing HeLa cells. (D) HEK293 cells were transiently transfected with MYC-RAB5A, and/or Twinstrep-CapZβ, and the cell lysates were incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblotting analysis against CapZβ and RAB5A. (E) HEK293 cells were transiently transfected with His6-CapZβ and/or Twinstrep-RAB5A, and the cell lysates were then incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblot analysis against CapZβ and RAB5A. (F) The lysates of Twinstrep-CapZβ-expressing HEK293 cells were incubated with GST or GST-RAB5A recombinant proteins in the presence of GDPβS or GTPγS, followed by GST pulldown and strep immunoblotting. The blots, images, and graphs represent data from at least three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm. MCC, Manders colocalization coefficient.
Capzβ, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody rabbit polyclonal igg anti-p34-arc/arpc2 (arp2/3 complex)  (Millipore)
90
Millipore antibody rabbit polyclonal igg anti-p34-arc/arpc2 (arp2/3 complex)
Figure 1. <t>CapZ</t> is associated with early endosomes. (A) CapZβ-GFP-expressing HeLa cells were immunostained <t>with</t> <t>antibodies</t> against EEA1 (top panel) or RAB5A (lower panel). Confocal images taken along the z-axis were projected (Maximum Intensity Projection). The colocalization coefficients (MCC) of CapZβ-GFP and EEA1 or CapZβ-GFP and RAB5A were quantified. (B) CapZβ-GFP/RFP-RAB5A-expressing HeLa cells were treated with or without YM-201636 (5 μM) for 8 hr, followed by confocal imaging. The colocalization coefficients (MCC) of mRFP-RAB5A and CapZβ-GFP were quantified. (C) The N-SIM S Super-resolution imaging of CapZβ-mCherry/RAB5A-GFP-expressing HeLa cells. (D) HEK293 cells were transiently transfected with MYC-RAB5A, and/or Twinstrep-CapZβ, and the cell lysates were incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblotting analysis against CapZβ and RAB5A. (E) HEK293 cells were transiently transfected with His6-CapZβ and/or Twinstrep-RAB5A, and the cell lysates were then incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblot analysis against CapZβ and RAB5A. (F) The lysates of Twinstrep-CapZβ-expressing HEK293 cells were incubated with GST or GST-RAB5A recombinant proteins in the presence of GDPβS or GTPγS, followed by GST pulldown and strep immunoblotting. The blots, images, and graphs represent data from at least three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm. MCC, Manders colocalization coefficient.
Antibody Rabbit Polyclonal Igg Anti P34 Arc/Arpc2 (Arp2/3 Complex), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti arp2 3  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti arp2 3
Figure 1. <t>CapZ</t> is associated with early endosomes. (A) CapZβ-GFP-expressing HeLa cells were immunostained <t>with</t> <t>antibodies</t> against EEA1 (top panel) or RAB5A (lower panel). Confocal images taken along the z-axis were projected (Maximum Intensity Projection). The colocalization coefficients (MCC) of CapZβ-GFP and EEA1 or CapZβ-GFP and RAB5A were quantified. (B) CapZβ-GFP/RFP-RAB5A-expressing HeLa cells were treated with or without YM-201636 (5 μM) for 8 hr, followed by confocal imaging. The colocalization coefficients (MCC) of mRFP-RAB5A and CapZβ-GFP were quantified. (C) The N-SIM S Super-resolution imaging of CapZβ-mCherry/RAB5A-GFP-expressing HeLa cells. (D) HEK293 cells were transiently transfected with MYC-RAB5A, and/or Twinstrep-CapZβ, and the cell lysates were incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblotting analysis against CapZβ and RAB5A. (E) HEK293 cells were transiently transfected with His6-CapZβ and/or Twinstrep-RAB5A, and the cell lysates were then incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblot analysis against CapZβ and RAB5A. (F) The lysates of Twinstrep-CapZβ-expressing HEK293 cells were incubated with GST or GST-RAB5A recombinant proteins in the presence of GDPβS or GTPγS, followed by GST pulldown and strep immunoblotting. The blots, images, and graphs represent data from at least three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm. MCC, Manders colocalization coefficient.
Anti Arp2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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regulation antibody sampler kit  (ECM Biosciences)
85
ECM Biosciences regulation antibody sampler kit
Figure 1. <t>CapZ</t> is associated with early endosomes. (A) CapZβ-GFP-expressing HeLa cells were immunostained <t>with</t> <t>antibodies</t> against EEA1 (top panel) or RAB5A (lower panel). Confocal images taken along the z-axis were projected (Maximum Intensity Projection). The colocalization coefficients (MCC) of CapZβ-GFP and EEA1 or CapZβ-GFP and RAB5A were quantified. (B) CapZβ-GFP/RFP-RAB5A-expressing HeLa cells were treated with or without YM-201636 (5 μM) for 8 hr, followed by confocal imaging. The colocalization coefficients (MCC) of mRFP-RAB5A and CapZβ-GFP were quantified. (C) The N-SIM S Super-resolution imaging of CapZβ-mCherry/RAB5A-GFP-expressing HeLa cells. (D) HEK293 cells were transiently transfected with MYC-RAB5A, and/or Twinstrep-CapZβ, and the cell lysates were incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblotting analysis against CapZβ and RAB5A. (E) HEK293 cells were transiently transfected with His6-CapZβ and/or Twinstrep-RAB5A, and the cell lysates were then incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblot analysis against CapZβ and RAB5A. (F) The lysates of Twinstrep-CapZβ-expressing HEK293 cells were incubated with GST or GST-RAB5A recombinant proteins in the presence of GDPβS or GTPγS, followed by GST pulldown and strep immunoblotting. The blots, images, and graphs represent data from at least three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm. MCC, Manders colocalization coefficient.
Regulation Antibody Sampler Kit, supplied by ECM Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti arp2 3 subunit 1b antibody  (Bioss)
86
Bioss rabbit anti arp2 3 subunit 1b antibody
ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and <t>Arp2/3</t> Western blotting results presented in A. ** p < 0.01.
Rabbit Anti Arp2 3 Subunit 1b Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti arpc4 proteintech rabbit anti-human arpc4  (Proteintech)
93
Proteintech anti arpc4 proteintech rabbit anti-human arpc4
ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and <t>Arp2/3</t> Western blotting results presented in A. ** p < 0.01.
Anti Arpc4 Proteintech Rabbit Anti Human Arpc4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti arp2 3 antibody  (Danaher Inc)
86
Danaher Inc rabbit polyclonal anti arp2 3 antibody
ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and <t>Arp2/3</t> Western blotting results presented in A. ** p < 0.01.
Rabbit Polyclonal Anti Arp2 3 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti arp2 3 antibody - by Bioz Stars, 2026-02
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rabbit anti brdu monoclonal antibody  (Boster Bio)
90
Boster Bio rabbit anti brdu monoclonal antibody
ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and <t>Arp2/3</t> Western blotting results presented in A. ** p < 0.01.
Rabbit Anti Brdu Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. CapZ is associated with early endosomes. (A) CapZβ-GFP-expressing HeLa cells were immunostained with antibodies against EEA1 (top panel) or RAB5A (lower panel). Confocal images taken along the z-axis were projected (Maximum Intensity Projection). The colocalization coefficients (MCC) of CapZβ-GFP and EEA1 or CapZβ-GFP and RAB5A were quantified. (B) CapZβ-GFP/RFP-RAB5A-expressing HeLa cells were treated with or without YM-201636 (5 μM) for 8 hr, followed by confocal imaging. The colocalization coefficients (MCC) of mRFP-RAB5A and CapZβ-GFP were quantified. (C) The N-SIM S Super-resolution imaging of CapZβ-mCherry/RAB5A-GFP-expressing HeLa cells. (D) HEK293 cells were transiently transfected with MYC-RAB5A, and/or Twinstrep-CapZβ, and the cell lysates were incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblotting analysis against CapZβ and RAB5A. (E) HEK293 cells were transiently transfected with His6-CapZβ and/or Twinstrep-RAB5A, and the cell lysates were then incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblot analysis against CapZβ and RAB5A. (F) The lysates of Twinstrep-CapZβ-expressing HEK293 cells were incubated with GST or GST-RAB5A recombinant proteins in the presence of GDPβS or GTPγS, followed by GST pulldown and strep immunoblotting. The blots, images, and graphs represent data from at least three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm. MCC, Manders colocalization coefficient.

Journal: eLife

Article Title: Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors

doi: 10.7554/elife.65910

Figure Lengend Snippet: Figure 1. CapZ is associated with early endosomes. (A) CapZβ-GFP-expressing HeLa cells were immunostained with antibodies against EEA1 (top panel) or RAB5A (lower panel). Confocal images taken along the z-axis were projected (Maximum Intensity Projection). The colocalization coefficients (MCC) of CapZβ-GFP and EEA1 or CapZβ-GFP and RAB5A were quantified. (B) CapZβ-GFP/RFP-RAB5A-expressing HeLa cells were treated with or without YM-201636 (5 μM) for 8 hr, followed by confocal imaging. The colocalization coefficients (MCC) of mRFP-RAB5A and CapZβ-GFP were quantified. (C) The N-SIM S Super-resolution imaging of CapZβ-mCherry/RAB5A-GFP-expressing HeLa cells. (D) HEK293 cells were transiently transfected with MYC-RAB5A, and/or Twinstrep-CapZβ, and the cell lysates were incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblotting analysis against CapZβ and RAB5A. (E) HEK293 cells were transiently transfected with His6-CapZβ and/or Twinstrep-RAB5A, and the cell lysates were then incubated with Strep-Tactin Sepharose. The pulldowns were subjected to immunoblot analysis against CapZβ and RAB5A. (F) The lysates of Twinstrep-CapZβ-expressing HEK293 cells were incubated with GST or GST-RAB5A recombinant proteins in the presence of GDPβS or GTPγS, followed by GST pulldown and strep immunoblotting. The blots, images, and graphs represent data from at least three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm. MCC, Manders colocalization coefficient.

Article Snippet: The primary antibodies were used as follows: GAPDH (Proteintech, 60004- 1- Ig), CapZβ (Proteintech, 25043- 1- AP), ARPC1B (Sigma- Aldrich, HPA004832), p16- Arc (Abcam, ab51243), N- WASP (Novus Biologicals, NBP1- 82512), His- tag (ProteinTech, 66005- 1- Ig), β-actin (ProteinTech, 60008- 1- Ig), α-tublin (ProteinTech, 66031- 1- Ig), VPS8 (ProteinTech, 15079- 1- AP), λ-tublin (Sigma- Aldrich, T6557), HSC70 (Santa Cruz, sc- 24), VPS34 (CST, 4263), CapZa1/a2 (Developmental Studies Hybridoma Bank, mAb 5B12.3), anti- pan actin (Cytoskeleton, AAN02- s), RAB5A (CST, 3547 or 46449), Calnexin (ProteinTech, 10427- 2- AP), LAMP1 (CST, 9091), TGN46 (Bio- Rad, AHP500GT), EEA1 (CST, 3288), MYC- tag (ProteinTech, 67447- 1- Ig), Flag- tag (Sigma- Aldrich, F3165), HA- tag (Sigma- Aldrich,11867423001), StrepMABClassic HRP conjugate (IBA, 2- 1509- 001), Rabex- 5 (Santa Cruz, sc- 166049), Rabaptin- 5 (ProteinTech, 14350- 1- AP), EGFR (Santa Cruz, sc- 373746), goat anti- mouse IgG (H+L) secondary antibody (Invitrogen, 31430), goat anti- rabbit IgG (H+L) secondary antibody (Invitrogen, 31460), and goat anti- rat IgG (H+L) secondary antibody (Invitrogen, 31470).

Techniques: Expressing, Imaging, Transfection, Incubation, Western Blot, Recombinant, Two Tailed Test

Figure 2. CapZ is required for early endosome maturation. (A) The expression of CapZβ in control, CapZβ-knockout, or CapZβ-reconstituted HeLa cells. (B) Control, CapZβ-knockout, or CapZβ-reconstituted HeLa cells were treated with EGF-488 for the indicated times, and then processed for immunofluorescence analysis. (C) Control or CapZβ-knockout HeLa cells were treated with EGF-488 for 15 min, and the number and size of EGF-positive vesicles in these cells were quantified. (D) Control, CapZβ-knockout, or CapZβ-reconstituted HeLa cells were immunostained with an anti-RAB5A antibody. The number and size of the early endosomes in these cells were quantified. (E) Active RAB5 in control or CapZβ knockout cells was examined with a GST-R5BD pulldown assay. The images and graphs represent data from three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm.

Journal: eLife

Article Title: Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors

doi: 10.7554/elife.65910

Figure Lengend Snippet: Figure 2. CapZ is required for early endosome maturation. (A) The expression of CapZβ in control, CapZβ-knockout, or CapZβ-reconstituted HeLa cells. (B) Control, CapZβ-knockout, or CapZβ-reconstituted HeLa cells were treated with EGF-488 for the indicated times, and then processed for immunofluorescence analysis. (C) Control or CapZβ-knockout HeLa cells were treated with EGF-488 for 15 min, and the number and size of EGF-positive vesicles in these cells were quantified. (D) Control, CapZβ-knockout, or CapZβ-reconstituted HeLa cells were immunostained with an anti-RAB5A antibody. The number and size of the early endosomes in these cells were quantified. (E) Active RAB5 in control or CapZβ knockout cells was examined with a GST-R5BD pulldown assay. The images and graphs represent data from three independent experiments. The data are expressed as mean ± SD. The difference between the two groups was analyzed using two-tailed Student’s t-test, p<0.05 was considered statistically significant. *** p<0.001. Scale bar, 5 μm.

Article Snippet: The primary antibodies were used as follows: GAPDH (Proteintech, 60004- 1- Ig), CapZβ (Proteintech, 25043- 1- AP), ARPC1B (Sigma- Aldrich, HPA004832), p16- Arc (Abcam, ab51243), N- WASP (Novus Biologicals, NBP1- 82512), His- tag (ProteinTech, 66005- 1- Ig), β-actin (ProteinTech, 60008- 1- Ig), α-tublin (ProteinTech, 66031- 1- Ig), VPS8 (ProteinTech, 15079- 1- AP), λ-tublin (Sigma- Aldrich, T6557), HSC70 (Santa Cruz, sc- 24), VPS34 (CST, 4263), CapZa1/a2 (Developmental Studies Hybridoma Bank, mAb 5B12.3), anti- pan actin (Cytoskeleton, AAN02- s), RAB5A (CST, 3547 or 46449), Calnexin (ProteinTech, 10427- 2- AP), LAMP1 (CST, 9091), TGN46 (Bio- Rad, AHP500GT), EEA1 (CST, 3288), MYC- tag (ProteinTech, 67447- 1- Ig), Flag- tag (Sigma- Aldrich, F3165), HA- tag (Sigma- Aldrich,11867423001), StrepMABClassic HRP conjugate (IBA, 2- 1509- 001), Rabex- 5 (Santa Cruz, sc- 166049), Rabaptin- 5 (ProteinTech, 14350- 1- AP), EGFR (Santa Cruz, sc- 373746), goat anti- mouse IgG (H+L) secondary antibody (Invitrogen, 31430), goat anti- rabbit IgG (H+L) secondary antibody (Invitrogen, 31460), and goat anti- rat IgG (H+L) secondary antibody (Invitrogen, 31470).

Techniques: Expressing, Control, Knock-Out, Immunofluorescence, Two Tailed Test

Figure 3. CapZ controls the actin density around early endosomes. (A, B) HeLa cells were stably transfected with EGFP/mCherry or CapZα1-EGFP/CapZβ-mCherry constructs. These cells were either immunostained with phalloidin (A), or with an anti-RAB5A antibody (B). The number and size of the early endosomes in these cells were quantified (B). (C) Control or CapZβ-knockout HeLa cells were immunostained with antibodies against actin and

Journal: eLife

Article Title: Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors

doi: 10.7554/elife.65910

Figure Lengend Snippet: Figure 3. CapZ controls the actin density around early endosomes. (A, B) HeLa cells were stably transfected with EGFP/mCherry or CapZα1-EGFP/CapZβ-mCherry constructs. These cells were either immunostained with phalloidin (A), or with an anti-RAB5A antibody (B). The number and size of the early endosomes in these cells were quantified (B). (C) Control or CapZβ-knockout HeLa cells were immunostained with antibodies against actin and

Article Snippet: The primary antibodies were used as follows: GAPDH (Proteintech, 60004- 1- Ig), CapZβ (Proteintech, 25043- 1- AP), ARPC1B (Sigma- Aldrich, HPA004832), p16- Arc (Abcam, ab51243), N- WASP (Novus Biologicals, NBP1- 82512), His- tag (ProteinTech, 66005- 1- Ig), β-actin (ProteinTech, 60008- 1- Ig), α-tublin (ProteinTech, 66031- 1- Ig), VPS8 (ProteinTech, 15079- 1- AP), λ-tublin (Sigma- Aldrich, T6557), HSC70 (Santa Cruz, sc- 24), VPS34 (CST, 4263), CapZa1/a2 (Developmental Studies Hybridoma Bank, mAb 5B12.3), anti- pan actin (Cytoskeleton, AAN02- s), RAB5A (CST, 3547 or 46449), Calnexin (ProteinTech, 10427- 2- AP), LAMP1 (CST, 9091), TGN46 (Bio- Rad, AHP500GT), EEA1 (CST, 3288), MYC- tag (ProteinTech, 67447- 1- Ig), Flag- tag (Sigma- Aldrich, F3165), HA- tag (Sigma- Aldrich,11867423001), StrepMABClassic HRP conjugate (IBA, 2- 1509- 001), Rabex- 5 (Santa Cruz, sc- 166049), Rabaptin- 5 (ProteinTech, 14350- 1- AP), EGFR (Santa Cruz, sc- 373746), goat anti- mouse IgG (H+L) secondary antibody (Invitrogen, 31430), goat anti- rabbit IgG (H+L) secondary antibody (Invitrogen, 31460), and goat anti- rat IgG (H+L) secondary antibody (Invitrogen, 31470).

Techniques: Stable Transfection, Transfection, Construct, Control, Knock-Out

Figure 4. The actin density around early endosomes regulates its maturation. (A) Control or CapZβ-knockout HeLa cells were treated with or without CK-636 (100 μM) overnight and were then immunostained with an anti-RAB5A antibody. The number and size of the early endosomes in these cells were quantified. (B) Control or ARP2-knockout HeLa cells were immunostained with antibodies against RAB5A and ARPC1B, followed by confocal imaging. The colocalization coefficients (MCC) of RAB5A and ARPC1B were quantified. The expression of ARP2 in these cells was analyzed by immunoblot

Journal: eLife

Article Title: Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors

doi: 10.7554/elife.65910

Figure Lengend Snippet: Figure 4. The actin density around early endosomes regulates its maturation. (A) Control or CapZβ-knockout HeLa cells were treated with or without CK-636 (100 μM) overnight and were then immunostained with an anti-RAB5A antibody. The number and size of the early endosomes in these cells were quantified. (B) Control or ARP2-knockout HeLa cells were immunostained with antibodies against RAB5A and ARPC1B, followed by confocal imaging. The colocalization coefficients (MCC) of RAB5A and ARPC1B were quantified. The expression of ARP2 in these cells was analyzed by immunoblot

Article Snippet: The primary antibodies were used as follows: GAPDH (Proteintech, 60004- 1- Ig), CapZβ (Proteintech, 25043- 1- AP), ARPC1B (Sigma- Aldrich, HPA004832), p16- Arc (Abcam, ab51243), N- WASP (Novus Biologicals, NBP1- 82512), His- tag (ProteinTech, 66005- 1- Ig), β-actin (ProteinTech, 60008- 1- Ig), α-tublin (ProteinTech, 66031- 1- Ig), VPS8 (ProteinTech, 15079- 1- AP), λ-tublin (Sigma- Aldrich, T6557), HSC70 (Santa Cruz, sc- 24), VPS34 (CST, 4263), CapZa1/a2 (Developmental Studies Hybridoma Bank, mAb 5B12.3), anti- pan actin (Cytoskeleton, AAN02- s), RAB5A (CST, 3547 or 46449), Calnexin (ProteinTech, 10427- 2- AP), LAMP1 (CST, 9091), TGN46 (Bio- Rad, AHP500GT), EEA1 (CST, 3288), MYC- tag (ProteinTech, 67447- 1- Ig), Flag- tag (Sigma- Aldrich, F3165), HA- tag (Sigma- Aldrich,11867423001), StrepMABClassic HRP conjugate (IBA, 2- 1509- 001), Rabex- 5 (Santa Cruz, sc- 166049), Rabaptin- 5 (ProteinTech, 14350- 1- AP), EGFR (Santa Cruz, sc- 373746), goat anti- mouse IgG (H+L) secondary antibody (Invitrogen, 31430), goat anti- rabbit IgG (H+L) secondary antibody (Invitrogen, 31460), and goat anti- rat IgG (H+L) secondary antibody (Invitrogen, 31470).

Techniques: Control, Knock-Out, Imaging, Expressing, Western Blot

ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and Arp2/3 Western blotting results presented in A. ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and Arp2/3 Western blotting results presented in A. ** p < 0.01.

Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R), rabbit anti-ARP2/3 subunit 1B antibody (bs-10563R), rabbit anti-CDC42 antibody (bs-3555R), rabbit anti-MyoD antibody (bs-2442R), rabbit anti-GAPDH antibody (bs-0755R) (all at dilution 1:500; Bioss Antibodies, China).

Techniques: Inhibition, Expressing, Western Blot

SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.

Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R), rabbit anti-ARP2/3 subunit 1B antibody (bs-10563R), rabbit anti-CDC42 antibody (bs-3555R), rabbit anti-MyoD antibody (bs-2442R), rabbit anti-GAPDH antibody (bs-0755R) (all at dilution 1:500; Bioss Antibodies, China).

Techniques: Western Blot, Activation Assay, Inhibition

SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R), rabbit anti-ARP2/3 subunit 1B antibody (bs-10563R), rabbit anti-CDC42 antibody (bs-3555R), rabbit anti-MyoD antibody (bs-2442R), rabbit anti-GAPDH antibody (bs-0755R) (all at dilution 1:500; Bioss Antibodies, China).

Techniques: Western Blot, Expressing, Activation Assay, Inhibition, Negative Control

Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.

Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R), rabbit anti-ARP2/3 subunit 1B antibody (bs-10563R), rabbit anti-CDC42 antibody (bs-3555R), rabbit anti-MyoD antibody (bs-2442R), rabbit anti-GAPDH antibody (bs-0755R) (all at dilution 1:500; Bioss Antibodies, China).

Techniques: Migration, Expressing